Dark microbiome and extremely low organics in Atacama fossil delta unveil Mars life detection limits

Armando Azua-Bustos (1, 2) , Alberto G. Fairén (1, 3) , Carlos González-Silva (4) , Olga Prieto-Ballesteros (1) , Daniel Carrizo (1) , Laura Sánchez-García (1) , Victor Parro (1) , Miguel Ángel Fernández-Martínez (5) , Cristina Escudero (1) , Victoria Muñoz-Iglesias (1) , Maite Fernández-Sampedro (1) , Antonio Molina (1) , Miriam García Villadangos (1) , Mercedes Moreno-Paz (1) , Jacek Wierzchos (6) , Carmen Ascaso (6) , Teresa Fornaro (7) , John Robert Brucato (7) , Giovanni Poggiali (7) , Jose Antonio Manrique (8, 9) , Marco Veneranda (8) , Guillermo López-Reyes (8) , Aurelio Sanz-Arranz (8) , Fernando Rull (8) , Ann M. Ollila (10) , Roger C. Wiens (10) , Adriana Reyes-Newell (11) , Samuel M. Clegg (10) , Maeva Millan (12, 13, 14) , Sarah Stewart Johnson (13, 15) , Ophélie Mcintosh (14) , Cyril Szopa (14) , Caroline Freissinet (14) , Yasuhito Sekine (16, 17) , Keisuke Fukushi (17) , Koki Morida (18) , Kosuke Inoue (18) , Hiroshi Sakuma (19) , Elizabeth Rampe (20)

1 CAB - Centro de Astrobiologia [Madrid]
2 ICB - Instituto de Ciencias Biomédicas [Santiago]
3 Department of Astronomy [Ithaca]
4 Universidad de Tarapaca
5 Departamento de Ecología [Madrid]
6 MNCN - Museo Nacional de Ciencias Naturales [Madrid]
7 OAA - INAF - Osservatorio Astrofisico di Arcetri
8 UVa - Universidad de Valladolid [Valladolid]
9 IRAP - Institut de recherche en astrophysique et planétologie
10 EAPS - Department of Earth, Atmospheric, and Planetary Sciences [West Lafayette]
11 Southwest Sciences, Inc.
12 GSFC Solar System Exploration Division
13 Department of Biology [Washington]
14 PLANETO - LATMOS
15 GU - Georgetown University [Washington]
16 ELSI - Earth-Life Science Institute [Tokyo]
17 Institute of Nature and Environmental [Kanazawa]
18 KU - Kanazawa University
19 NIMS - National Institute for Materials Science
20 JSC - NASA Johnson Space Center

Identifying unequivocal signs of life on Mars is one of the most important objectives for sending missions to the red planet. Here we report Red Stone, a 163-100 My alluvial fan–fan delta that formed under arid conditions in the Atacama Desert, rich in hematite and mudstones containing clays such as vermiculite and smectites, and therefore geologically analogous to Mars. We show that Red Stone samples display an important number of microorganisms with an unusual high rate of phylogenetic indeterminacy, what we refer to as “dark microbiome”, and a mix of biosignatures from extant and ancient microorganisms that can be barely detected with state-of-the-art laboratory equipment. Our analyses by testbed instruments that are on or will be sent to Mars unveil that although the mineralogy of Red Stone matches that detected by ground-based instruments on the red planet, similarly low levels of organics will be hard, if not impossible to detect in Martian rocks depending on the instrument and technique used. Our results stress the importance in returning samples to Earth for conclusively addressing whether life ever existed on Mars.

Domains: Sciences of the Universe [physics]

Armando Azua-Bustos, Alberto G. Fairén, Carlos González-Silva, Olga Prieto-Ballesteros, Daniel Carrizo, et al.. Dark microbiome and extremely low organics in Atacama fossil delta unveil Mars life detection limits. Nature Communications, 2023, 14, pp.808. ⟨10.1038/s41467-023-36172-1⟩. ⟨insu-04005234⟩