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An easy spectrophotometric acid-base titration protocol for dissolved organic matter

Abstract : UV-vis spectrophotometric acid-base titration can characterize dissolved organic matter (DOM) acid-base properties. However, it requires incremental pH adjustment, which make the procedure time consuming and results are subjected to dilution effect. This study brings forth a new approach, referred as the “buffer method” for pH adjustments by using carefully selective pH-buffers to adjust the pH. This, statistically validated method minimizes the pH adjustment time and lightens the laboratory work load. Chemical product cost associated with this novel method is slightly increased as compared to the previous approach, due to the necessity to use pH-buffers.• Buffer method: Acid-base titration by using buffer for pH adjustment• Buffer method validated by statistical means• Rapid, reliable and economical method
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Submitted on : Wednesday, May 11, 2022 - 9:01:25 AM
Last modification on : Thursday, June 2, 2022 - 2:48:18 PM


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Marawit Tesfa, Aline Dia, Malou Ollivier, Iván David Osorio Leon, Remi Marsac. An easy spectrophotometric acid-base titration protocol for dissolved organic matter. MethodsX, Elsevier, 2022, 9, pp.101721. ⟨10.1016/j.mex.2022.101721⟩. ⟨insu-03664468⟩



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