Investigation of Spin Coating Cerium-Doped Hydroxyapatite Thin Films with Antifungal Properties

Simona Liliana Iconaru (1) , Mihai Valentin Predoi (2) , Patrick Chapon (3) , Sofia Gaiaschi (3) , Krzysztof Rokosz (4) , Steinar Raaen (5) , Mikael Motelica-Heino (6, 7) , Daniela Predoi (1)

1 National Institute of Physics of Materials
2 UPB - University Politehnica of Bucharest [Romania]
3 HORIBA Jobin Yvon S.A.S
4 Koszalin University of Technology
5 NTNU - Norwegian University of Science and Technology [Aalesund]
6 ISTO - Institut des Sciences de la Terre d'Orléans - UMR7327
7 Biogéosystèmes Continentaux - UMR7327

In this study, the cerium-doped hydroxyapatite (Ca10−xCex(PO4)6(OH)2 with xCe = 0.1, 10Ce-HAp) coatings obtained by the spin coating method were presented for the first time. The stability of the 10Ce-HAp suspension particles used in the preparation of coatings was evaluated by ultrasonic studies, transmission electron microscopy (TEM), X-ray diffraction (XRD), and scanning electron microscopy (SEM). The surface morphology of the 10Ce-HAp coating was studied by SEM and atomic force microscopy (AFM) techniques. The obtained 10Ce-HAp coatings were uniform and without cracks or unevenness. Glow discharge optical emission spectroscopy (GDOES) and X-ray photoelectron spectroscopy (XPS) were used for the investigation of fine chemical depth profiling. The antifungal properties of the HAp and 10Ce-HAp suspensions and coatings were assessed using Candida albicans ATCC 10231 (C. albicans) fungal strain. The quantitative antifungal assays demonstrated that both 10Ce-HAp suspensions and coatings exhibited strong antifungal properties and that they successfully inhibited the development and adherence of C. albicans fungal cells for all the tested time intervals. The scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) visualization of the C. albicans fungal cells adherence to the 10Ce-HAp surface also demonstrated their strong inhibitory effects. In addition, the qualitative assays also suggested that the 10Ce-HAp coatings successfully stopped the biofilm formation.

Keywords: cerium; hydroxyapatite; spin coating; antifungal properties

Domains: Sciences of the Universe [physics]; Sciences of the Universe [physics]; Continental interfaces, environment

Simona Liliana Iconaru, Mihai Valentin Predoi, Patrick Chapon, Sofia Gaiaschi, Krzysztof Rokosz, et al.. Investigation of Spin Coating Cerium-Doped Hydroxyapatite Thin Films with Antifungal Properties. Coatings, 2021, 11 (4), pp.464. ⟨10.3390/coatings11040464⟩. ⟨insu-03202144⟩