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High Precision Zinc Isotopic Measurements Applied to Mouse Organs

Abstract : We present a procedure to measure with high precision zinc isotope ratios in mouse organs. Zinc is composed of 5 stable isotopes (Zn-64, Zn-66, Zn-67, Zn-68 and Zn-70) which are naturally fractionated between mouse organs. We first show how to dissolve the different organs in order to free the Zn atoms; this step is realized by a mixture of HNO3 and H2O2. We then purify the zinc atoms from all the other elements, in particular from isobaric interferences (e.g., Ni), by anion-exchange chromatography in a dilute HBr/HNO3 medium. These first two steps are performed in a clean laboratory using high purity chemicals. Finally, the isotope ratios are measured by using a multi-collector inductively-coupled-plasma mass-spectrometer, in low resolution. The samples are injected using a spray chamber and the isotopic fractionation induced by the mass-spectrometer is corrected by comparing the ratio of the samples to the ratio of a standard (standard bracketing technique). This full typical procedure produces an isotope ratio with a 50 ppm (2 s.d.) reproducibility.
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Submitted on : Wednesday, August 19, 2020 - 3:39:35 PM
Last modification on : Thursday, April 7, 2022 - 1:58:31 PM

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Frédéric Moynier, Marie Le Borgne. High Precision Zinc Isotopic Measurements Applied to Mouse Organs. Journal of visualized experiments : JoVE, JoVE, 2015, ⟨10.3791/52479⟩. ⟨insu-02917635⟩

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