Skip to Main content Skip to Navigation
Journal articles

Application of TMAH thermochemolysis to the detection of nucleobases: Application to the MOMA and SAM space experiment

Abstract : Thermochemolysis of seven nucleobases—adenine, thymine, uracil, cytosine, guanine, xanthine, and hypoxanthine—in tetramethylammonium hydroxide (TMAH) was studied individually by pyrolysis gas chromatography mass spectrometry in the frame of the Mars surface exploration. The analyses were performed under conditions relevant to the Sample Analysis at Mars (SAM) instrument of the Mars Curiosity Rover and the Mars Organic Molecule Analyzer (MOMA) instrument of the ExoMars Rover. The thermochemolysis products of each nucleobase were identified and the reaction mechanisms studied. The thermochemolysis temperature was optimized and the limit of detection and quantification of each nucleobase were also investigated. Results indicate that 600°C is the optimal thermochemolysis temperature for all seven nucleobases. The methylated products trimethyl-adenine, 1, 3-dimethyl-thymine, 1, 3-dimethyl-uracil, trimethyl-cytosine, 1, 3, 7-trimethyl-xanthine (caffeine), and dimethyl-hypoxanthine, respectively, are the most stable forms of adenine, thymine, uracil, cytosine, guanine, and xanthine, and hypoxanthine in TMAH solutions. The limits of detection for adenine, thymine, and uracil were 0.075 nmol; the limits of detection for guanine, cytosine, and hypoxanthine were higher, at 0.40, 0.55, and 0.75 nmol, respectively. These experiments allowed to well constrain the analytical capabilities of the thermochemolysis experiments that will be performed on Mars to detect nucleobases.
Document type :
Journal articles
Complete list of metadata
Contributor : Accord Elsevier CCSD Connect in order to contact the contributor
Submitted on : Monday, October 25, 2021 - 1:10:01 PM
Last modification on : Monday, August 8, 2022 - 5:38:05 PM
Long-term archiving on: : Wednesday, January 26, 2022 - 8:01:27 PM


Files produced by the author(s)


Distributed under a Creative Commons Attribution - NonCommercial 4.0 International License



Y. He, Arnaud Buch, M. Morisson, Cyril Szopa, Caroline Freissinet, et al.. Application of TMAH thermochemolysis to the detection of nucleobases: Application to the MOMA and SAM space experiment. Talanta, Elsevier, 2019, 204, pp.802-811. ⟨10.1016/j.talanta.2019.06.076⟩. ⟨insu-02165608⟩



Record views


Files downloads