Toxicity Evaluation following Intratracheal Instillation of Iron Oxide in a Silica Matrix in Rats

Abstract : Iron oxide-silica nanoparticles (IOSi-NPs) were prepared from a mixture of ferrous chloride tetrahydrate and ferric chloride hexahydrate dropped into a silica xerogel composite. The structure and morphology of the synthesized maghemite nanoparticles into the silica xerogel were analysed by X-ray diffraction measurements, scanning electron microscopy equipped with an energy dispersive X-ray spectrometer, and transmission electron microscopy.Theresults of theEDAXanalysis indicated that the embedded particles were iron oxide nanoparticles. The particle size of IOSi-NPs calculated from the XRD analysis was estimated at around 12.5 nm.Theaverage size deduced fromthe particle size distribution is 13.7 ± 0.6 nm,which is in good agreementwith XRDanalysis. The biocompatibility of IOSi-NPs was assessed by cell viability and cytoskeleton analysis. Histopathology analysis was performed after 24 hours and 7 days, respectively, fromthe intratracheal instillation of a solution containing 0.5, 2.5, or 5mg/kg IOSi-NPs. The pathological micrographs of lungs derived fromrats collected after the intratracheal instillationwith a solution containing 0.5mg/kg and 2.5mg/kg IOSi-NPs show that the lung has preserved the architecture of the control specimen with no significant differences. However, even at concentrations of 5mg/kg, the effect of IOSi-NPS on the lungs was markedly reduced at 7 days posttreatment.
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Alina Mihaela Prodan, Carmen Steluta Ciobanu, Cristina Popa, Simona Liliana Iconaru, Daniela Predoi. Toxicity Evaluation following Intratracheal Instillation of Iron Oxide in a Silica Matrix in Rats. BioMed Research International , Hindawi Publishing Corporation, 2014, 13 p. ⟨10.1155/2014/134260⟩. ⟨insu-01061030⟩

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