Systematic investigation and in vitro biocompatibility studies on mesoporous europium doped hydroxyapatite

Cristina L. Popa (1) , Carmen Steluta Ciobanu (1) , Simona Liliana Iconaru (2, 3) , Miruna Stan (4) , Anca Dinischiotu (4) , Constantin C. Negrila (1) , Mikael Motelica-Heino (3) , Régis Guégan (3) , Daniela Predoi (1)

1 National Institute of Materials Physics
2 National Institute of Materials Physics
3 ISTO - Institut des Sciences de la Terre d'Orléans - UMR7327
4 Department of Biochemistry and Molecular Biology

This paper reports a systematic investigation on europium doped hydroxyapatite (Eu:HAp). In this work, a set of complementary techniques Fourier Transform Infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM) and, Brunauer-Emmett-Teller (BET) technique was used to allowing a proper understanding of Eu:HAp. The XPS analysis confirmed the substitution of Ca ions by Eu ions in Eu:HAp samples. Eu:HAp and pure HAp show the isotherms of type IV with a hysteresis loop at a relative pressure (P/P0) between 0.4 and 1.0, indicating the presence of mesopores. Finally, the in vitro biological effects of Eu:HAp nanoparticles were evaluated by focusing on F-actin filaments pattern and heat shock proteins (Hsp) expression in HEK293 human kidney cells. Fluorescence microscopy studies of the actin protein revealed no changes of the immunolabeling profile in the renal cells cultured in the presence of Eu:HAp nanoparticles. Hsp60, Hsp70 and Hsp90 expression measured by Western blot analysis were not affected after a 24 and 48 hours exposure. These results confirmed the lack of nanoparticles' toxicity and the biocompatibility of Eu:HAp nanoparticles and their possibility possible uses of using them in medical purposes without affecting the renal function.

Keywords: Nanoparticles; Hydroxyapatite; Europium; In vitro studies

Domains: Continental interfaces, environment; Global Changes

Cristina L. Popa, Carmen Steluta Ciobanu, Simona Liliana Iconaru, Miruna Stan, Anca Dinischiotu, et al.. Systematic investigation and in vitro biocompatibility studies on mesoporous europium doped hydroxyapatite. Central European Journal of Chemistry, 2014, 12 (10), pp.1032-1046. ⟨10.2478/s11532-014-0554-y⟩. ⟨insu-01017548⟩