Journal articles
Larval midgut modifications associated with Bti resistance in the yellow fever mosquito using proteomic and transcriptomic approaches
Abstract : Background: Bacillus thuringiensis var. israelensis ( Bti ) is a natural larval mosquito pathogen producing pore-forming toxins targeting the midgut of Diptera larvae. It is used worldwide for mosquito control. Resistance mechanisms of an Aedes aegypti laboratory strain selected for 30 generations with field-collected leaf litter containing Bti toxins were investigated in larval midguts at two levels: 1. gene transcription using DNA microarray and RT-qPCR and 2. differential expression of brush border membrane proteins using DIGE (Differential In Gel Electrophoresis). Results: Several Bti Cry toxin receptors including alkaline phosphatases and N-aminopeptidases and toxin-binding V-ATPases exhibited altered expression levels in the resistant strain. The under-expression of putative Bti- receptors is consistent with Bt -resistance mechanisms previously described in Lepidoptera. Four soluble metalloproteinases were found under-transcribed together with a drastic decrease of metalloproteinases activity in the resistant strain, suggesting a role in resistance by decreasing the amount of activated Cry toxins in the larval midgut. Conclusions: By combining transcriptomic and proteomic approaches, we detected expression changes at nearly each step of the ingestion-to-infection process, providing a short list of genes and proteins potentially involved in Bti -resistance whose implication needs to be validated. Collectively, these results open the way to further functional analyses to better characterize Bti- resistance mechanisms in mosquitoes.
Cited literature [84 references]
https://hal-insu.archives-ouvertes.fr/insu-00845245
Contributor : Pascale Talour Connect in order to contact the contributor
Submitted on : Tuesday, July 16, 2013 - 4:49:05 PM
Last modification on : Monday, December 7, 2020 - 10:12:01 AM
Contributor : Pascale Talour Connect in order to contact the contributor
Submitted on : Tuesday, July 16, 2013 - 4:49:05 PM
Last modification on : Monday, December 7, 2020 - 10:12:01 AM
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1471-2164-13-248.pdf
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