Journal articles
Structure, Orientation and Stability of Lysozyme Confined in layered Materials
Abstract : The confinement of lysozyme in 3 layered materials based on montmorillonite and lamellar double hydroxide exhibiting different surface charges was studied. The protein structure and orientation in these materials were determined by X-Ray diffraction, time resolved fluorescence and fluorescence anisotropy. For both Montmorillonite exchanged with sodium and modified with non-ionic surfactant (tri-ethylene glycol mono n-decyl ether), the lysozyme was found to be located in the interlayer space with "end-on" and "side-on" orientation, respectively. Conversely no lysozyme intercalation was observed with lamellar double hydroxide modified with anionic surfactant (sodium octylsulfate), since protein was adsorbed on the surface of the particles. Fourier transformed infra-red spectroscopy analysis shows that lysozyme confinement in the interlayer space preserves its structure after dehydration, whereas some structural changes were observed for lysozyme adsorbed on the particle surface.
Cited literature [11 references]
https://hal-insu.archives-ouvertes.fr/insu-00779421
Contributor : Nathalie Pothier <>
Submitted on : Wednesday, February 20, 2013 - 10:48:12 AM
Last modification on : Tuesday, December 8, 2020 - 3:45:17 AM
Long-term archiving on: : Sunday, April 2, 2017 - 2:43:04 AM
Contributor : Nathalie Pothier <>
Submitted on : Wednesday, February 20, 2013 - 10:48:12 AM
Last modification on : Tuesday, December 8, 2020 - 3:45:17 AM
Long-term archiving on: : Sunday, April 2, 2017 - 2:43:04 AM
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