Skip to Main content Skip to Navigation
Journal articles

An appropriate selection of 3D alginate culture model for hepatic Huh-7 cell line encapsulation intended for viral studies

Abstract : Three-dimensional (3D) culture systems have been introduced to provide cells with a biomimetic environment similar to in vivo conditions. Among the polymeric molecules available, sodium alginate salt (Na-alg) is a material currently employed in different areas of drug delivery and tissue engineering because it offers biocompatibility, optimal chemical properties and its gelation with calcium chloride provides calcium alginate (Ca-alg) scaffolds with mechanical stability and relative permeability. In this work, four different preparations of Ca-alg beads with varying Na-alg viscosity and concentration were used for a human hepatoma cell line (Huh-7) encapsulation. The effects of Ca-alg bead preparation on structural cell organization, liver-specific functions, and specific receptors expression implicated in hepatotropic virus permissivity were evaluated. Hepatic cells were cultured in 500Am diameter Ca-alg beads during 7 days under dynamic condition. For all culture systems, cell viability reached almost 100% at day 7. Cell proliferation was concomitantly followed by hepatocyte organization in aggregates, which adopted two different morphologies (spheroïd aggregates or multicellular channel-like structures), depending on Ca-alg bead preparation. These cellular organizations established real 3D hepatocyte architecture with cell polarity, cell junctions and abundant bile canaliculi possessing microvillus-lined channels. The functionality of these 3D cultures was confirmed by the production of albumin and the exhibition of activity CYP1A over culture time, which were variable, according to Ca-alg bead condition. The expression of specific receptors of Hepatitis C Virus (HCV) by Huh-7 cells suggests encouraging data for further development of a new viral culture system in Ca-alg beads. In summary, this 3D hepatic cell culture represents a promising physiologically relevant system for further in vitro studies and demonstrates that adequate encapsulation condition can be selected for each target application in liver tissue engineering, specifically in viral studies.
Complete list of metadatas

https://hal-insu.archives-ouvertes.fr/insu-00723045
Contributor : Nathalie Pothier <>
Submitted on : Tuesday, August 7, 2012 - 10:59:12 AM
Last modification on : Wednesday, October 14, 2020 - 3:58:09 AM

Identifiers

Collections

Citation

N.M. Tran, M. Dufresne, Gilles Duverlie, Sandrine Castelain, Christian Défarge, et al.. An appropriate selection of 3D alginate culture model for hepatic Huh-7 cell line encapsulation intended for viral studies. Tissue Engineering: Parts A, B, and C, Mary Ann Liebert, 2013, 19 (1-2), pp.103-113. ⟨10.1089/ten.tea.2012.0139⟩. ⟨insu-00723045⟩

Share

Metrics

Record views

344