Skip to Main content Skip to Navigation
Journal articles

Functional mechanisms of the cellular prion protein (PrPC) associated anti-HIV-1 properties

Abstract : The cellular prion protein PrPC/CD230 is a GPI-anchor protein highly expressed in cells from the nervous and immune systems and well conserved among vertebrates. In the last decade, several studies suggested that PrPC displays antiviral properties by restricting the replication of different viruses, and in particular retroviruses such as murine leukemia virus (MuLV) and the human immunodeficiency virus type 1 (HIV-1). In this context, we previously showed that PrPC displays important similarities with the HIV-1 nucleocapsid protein and found that PrPC expression in a human cell line strongly reduced HIV-1 expression and virus production. Using different PrPC mutants, we report here that the anti-HIV-1 properties are mostly associated with the amino-terminal 24-KRPKP-28 basic domain. In agreement with its reported RNA chaperone activity, we found that PrPC binds to the viral genomic RNA of HIV-1 and negatively affects its translation. Using a combination of biochemical and cell imaging strategies, we found that PrPC colocalizes with the virus assembly machinery at the plasma membrane and at the virological synapse in infected T cells. Depletion of PrPC in infected T cells and microglial cells favors HIV-1 replication, confirming its negative impact on the HIV-1 life cycle
Complete list of metadatas

https://hal-insu.archives-ouvertes.fr/insu-00696948
Contributor : Margaret Lartaud <>
Submitted on : Monday, May 14, 2012 - 11:17:52 AM
Last modification on : Tuesday, July 21, 2020 - 3:59:19 AM

Links full text

Identifiers

Citation

Sandrine Alais, Ricardo Soto-Rifo, Vincent Balter, Henri Gruffat, Evelyne Manet, et al.. Functional mechanisms of the cellular prion protein (PrPC) associated anti-HIV-1 properties. Cellular and Molecular Life Sciences, Springer Verlag, 2012, 69 (8), pp.1331-1352. ⟨10.1007/s00018-011-0879-z⟩. ⟨insu-00696948⟩

Share

Metrics

Record views

399