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Article Dans Une Revue (Data Paper) Acta Neuropathologica Année : 2017

A driver role for GABA metabolism in controlling stem and proliferative cell state through GHB production in glioma

Laurent Turchi
Bryan M Wittmann
  • Fonction : Auteur
Ghislaine Morvan-Dubois
Johan Pallud
Pascale Varlet
Isabelle Plo
Thierry Virolle
  • Fonction : Auteur
  • PersonId : 853566

Résumé

Cell populations with differing proliferative, stem-like and tumorigenic states co-exist in most tumors and especially malignant gliomas. Whether metabolic variations can drive this heterogeneity by controlling dynamic changes in cell states is unknown. Metabolite profiling of human adult glioblastoma stem-like cells upon loss of their tumorigenicity revealed a switch in the catabolism of the GABA neurotransmitter toward enhanced production and secretion of its by-product GHB (4-hydroxybutyrate). This switch was driven by succinic semialdehyde dehydrogenase (SSADH) downregulation. Enhancing GHB levels via SSADH downregulation or GHB supplementation triggered cell conversion into a less aggressive phenotypic state. GHB affected adult glioblastoma cells with varying molecular profiles, along with cells from pediatric pontine gliomas. In all cell types, GHB acted by inhibiting α-ketoglutarate-dependent Ten–eleven Translocations (TET) activity, resulting in decreased levels of the 5-hydroxymethylcytosine epigenetic mark. In patients, low SSADH expression was correlated with high GHB/α- ketoglutarate ratios, and distinguished weakly proliferative/ differentiated glioblastoma territories from proliferative/ non-differentiated territories. Our findings support an active participation of metabolic variations in the genesis of tumor heterogeneity.

Domaines

Cancer
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Origine : Publication financée par une institution

Dates et versions

inserm-01481051 , version 1 (02-03-2017)

Identifiants

Citer

Elias A El-Habr, Gustavo Luiz Dubois, Fanny Burel-Vandenbos, Alexandra Bogeas, Joanna Lipecka, et al.. A driver role for GABA metabolism in controlling stem and proliferative cell state through GHB production in glioma. Acta Neuropathologica, 2017, 133 (4), pp.645-660. ⟨10.1007/s00401-016-1659-5⟩. ⟨inserm-01481051⟩
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