Extracellular matrix-mimetic composite hydrogels of cross-linked hyaluronan and fibrillar collagen with tunable properties and ultrastructure - Matériaux et Biolologie Accéder directement au contenu
Article Dans Une Revue Carbohydrate Polymers Année : 2020

Extracellular matrix-mimetic composite hydrogels of cross-linked hyaluronan and fibrillar collagen with tunable properties and ultrastructure

Résumé

A platform of enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels with tunable compositions and gelation conditions was developed to evaluate the impact of the preparation conditions on their physical, chemical and biological properties. At low HA-Tyr content, hydrogels exhibited a fibrillar structure, with lower mechanical properties compared to pure Col hydrogels. At high HA-Tyr and Horse Radish Peroxydase (HRP) content, a microfibrillar network was formed beside the banded Col fibrils and a synergistic effect of the hybrid structure on mechanical properties was observed. These hydrogels were highly resistant against enzymatic degradation while keeping a high degree of hydration. Unlike HA-Tyr hydrogels, encapsulation of human dermal fibroblasts within Col/HA-Tyr hydrogels allowed for high cell viability. These results show that high HA-Tyr and HRP concentrations are required to positively impact the physical properties of hydrogels while preserving collagen fibrils. Those Col/HA-Tyr hydrogels appear promising for novel tissue engineering applications following a biomimetic approach.

Domaines

Chimie
Fichier principal
Vignette du fichier
CARBPOL-D-19-04819_R1-Christophe Hélary 2020.pdf (10.15 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-02862482 , version 1 (09-06-2020)

Identifiants

Citer

Antoine Frayssinet, Dalila Petta, Corinne Illoul, Bernard Haye, Anastasiia Markitantova, et al.. Extracellular matrix-mimetic composite hydrogels of cross-linked hyaluronan and fibrillar collagen with tunable properties and ultrastructure. Carbohydrate Polymers, 2020, 236, pp.116042. ⟨10.1016/j.carbpol.2020.116042⟩. ⟨hal-02862482⟩
140 Consultations
153 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More