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Theses

Sensitive and mutiplexed microRNA quantification using amplified time-gated Förster resonance energy transfer

Abstract : As new generation of biomarkers, microRNAs are associated with many cancers and diseases, which has led to a great demand for developing clinical miRNA diagnostic methods. Isothermal amplification technologies, such as rolling circle amplification and catalytic hairpin assembly, have emerged as powerful methods for highly rapid, specific and sensitive microRNA assays. This thesis focuses on developing microRNA biosensors based on isothermal amplification technologies and time-resolved Förster resonance energy transfer from lanthanide complexes to organic dyes or quantum dots. The proposed amplified microRNA biosensors have very low limits of detections, and are applied to human clinical samples, successfully revealing the relevance for cancer diagnostics. As simultaneous detection of multiple microRNAs is highly demanded, temporal multiplexed detection of microRNAs is also realized based on distinguishable excited-state lifetimes of Tb complexes and dyes. Moreover, the amplified microRNA nanosensor based on Tb-to-quantum dots FRET demonstrated the possibility of spectral multiplexed detection of microRNAs with high sensitivity and selectivity.
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Submitted on : Tuesday, September 1, 2020 - 10:13:08 AM
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  • HAL Id : tel-02926834, version 1

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Jingyue Xu. Sensitive and mutiplexed microRNA quantification using amplified time-gated Förster resonance energy transfer. Optics [physics.optics]. Université Paris-Saclay, 2020. English. ⟨NNT : 2020UPASS137⟩. ⟨tel-02926834⟩

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