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Article Dans Une Revue Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy [1994-...] Année : 2022

Determination of dissolved nickel in natural waters using a rapid microplate fluorescence assay method

Résumé

A new microplate analytical procedure is described for the determination of nickel (Ni2+) ions in natural water samples. A lophine analogue fluorescent sensor was synthesized and a spectral study showed a selective fluorescence quenching effect of chemical sensor by Ni2+ under optimized conditions. Density functional theory (DFT) calculations confirmed the formation of a Ni(II)L3 complex obtained by the Job plot. The calculations showed that the fluorescence emission peak of L collapses due to the distortion of L in the complex. The simple and fast microplate procedure allowed us to quantify Ni2+ with a linear response from 1.6 to 40 µg L-1 and a quantification limit of 5.4 µg L-1 without the need of a preconcentration step. The optimized procedure using high-throughput microplate assay has been applied for the determination of Ni 2+ in natural water samples with good analytical performances.
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Dates et versions

hal-03621175 , version 1 (28-03-2022)

Identifiants

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Fabien Robert-Peillard, El Mountassir El Mouchtari, Damien Bonne, Stéphane Humbel, Jean-Luc Boudenne, et al.. Determination of dissolved nickel in natural waters using a rapid microplate fluorescence assay method. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy [1994-..], 2022, 275, pp.121170. ⟨10.1016/j.saa.2022.121170⟩. ⟨hal-03621175⟩
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