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| HAL : hal-00376039, version 1 |
| PubMed : 15968115 |
| DOI : 10.1107/S0909049505009222 |
| Fiche détaillée |  Récupérer au format |
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| Journal of Synchrotron Radiation 12, Pt 4 (2005) 402-9 |
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| X-ray spectroscopy and X-ray diffraction at wavelengths near the K-absorption edge of phosphorus. |
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| Valérie Biou 1Peter Bösecke |
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| (07/2005) |
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| Phosphorus is an abundant element in living organisms. It is traceable by its X-ray absorption spectrum which shows a strong white line at its K-edge, comparable with that observed for the L(III) edges of rare earth ions. With purple membrane, the variation of the imaginary part of the anomalous dispersion of phosphorus is found to be close to 20 anomalous electron units. Anomalous diffraction experiments at wavelengths near the K-absorption edge of phosphorus confirm this result. The spatial distribution of lipids derived from anomalous diffraction agrees with earlier results from neutron diffraction. Test experiments on single crystals of the carrier protein using 5.76 A photons gave a first low-resolution diffraction pattern. Various techniques of crystal mounting were attempted. In addition, fluorescence measurements on a solution of threonine synthase appear to hint at a change of the phosphate environment of the cofactor upon activator binding. |
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| 1 : | Laboratoire d'enzymologie et biochimie structurales (LEBS) |
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CNRS : UPR9063 |
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| 2 : | Laboratoire de Mathématiques (LM-Reims) |
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CNRS : UMR6056 – Université de Reims - Champagne Ardenne |
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| 3 : | Biochimie et biophysique des systèmes intégrés (BBSI) |
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CNRS : UMR5092 – CEA : DSV/IRTSV – Université Joseph Fourier - Grenoble I |
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| 4 : | inconnu |
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Inconnu |
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| 5 : | Laboratory for Atmospheres |
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NASA Goddard Space Flight Center |
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| 6 : | Geophysical Jet Propulsion Laboratory |
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Geophysical Jet Propulsion Laboratory |
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| 7 : | Centre Laser Franco Allemand (CLFA) |
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Aucune |
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| 8 : | Synthèse et étude de systèmes à intêret biologique (SEESIB) |
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CNRS : UMR6504 – Université Blaise Pascal - Clermont-Ferrand II |
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| 9 : | Laboratoire des Protéines Membranaires (LPM) |
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CEA : DSV/IBS |
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| 10 : | Laboratoire de Microscopie Moléculaire et Cellulaire |
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Institut Gustave Roussy – CNRS : UMR1598 – Université Paris XI - Paris Sud |
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| 11 : | Institut des Sciences de la Terre d'Orléans (ISTO) |
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CNRS : UMR6113 – INSU – Université d'Orléans |
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| 12 : | Institut de biologie structurale (IBS) |
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CNRS : UMR5075 – CEA : DSV/IBS – Université Joseph Fourier - Grenoble I |
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| Domaine | : | Sciences du Vivant/Biochimie, Biologie Moléculaire/Biochimie |
| hal-00376039, version 1 | |
| http://hal.archives-ouvertes.fr/hal-00376039 | |
| oai:hal.archives-ouvertes.fr:hal-00376039 | |
| Contributeur : Gérard Brandolin | |
| Soumis le : Jeudi 16 Avril 2009, 15:39:21 | |
| Dernière modification le : Jeudi 16 Avril 2009, 15:39:21 | |
