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| HAL : hal-00282496, version 1 |
| PubMed : 16792589 |
| DOI : 10.1111/j.1398-9995.2006.01065.x |
| Fiche détaillée |  Récupérer au format |
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| Allergy 61, 7 (2006) 886-90 |
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| Dexamethasone and IL-10 stimulate glucocorticoid-induced leucine zipper synthesis by human mast cells. |
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| V. Godot 1G. Garcia 2, 3, 4, 5 |
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| (07/2006) |
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| BACKGROUND: Glucocorticoids (GCs) decrease tissue mast cell (MC) number and prevent their activation via their high-affinity IgE receptor. Glucocorticoid-induced leucine zipper (GILZ) is one of the GC-induced genes, which inhibits the functions of the transcriptional activators AP-1 and NF-kappaB. GILZ appears to be a critical actor in the anti-inflammatory and immunosuppressive effects of GCs in human T lymphocytes, macrophages and dendritic cells. AIMS OF THE STUDY: We investigated whether GILZ was produced by human MCs and whether GILZ synthesis was stimulated by GCs. We also investigated whether GILZ production was modulated by (i) IL-10, because of its common immunosuppressive properties with GCs, (ii) histamine because of its pro-inflammatory properties and (iii) IL-4 and IL-5 because of their ability to favour MC survival and proliferation with SCF. METHODS: The human MC lines HMC-1 5C6 and LAD-2, and cord blood-derived MCs (CB-MCs) were cultured alone or in the presence of GCs, IL-10, histamine, IL-4 or IL-5. The expression of GILZ was evaluated by using RT-PCR, Western blotting or immunocytochemistry. RESULTS: We found that human MC lines and CB-MCs constitutively produce GILZ. We also show that GCs and IL-10 stimulate GILZ production by human MCs. Our present results indicate that histamine, IL-4 and IL-5 alone or in combination with SCF do not downregulate GILZ production by MCs. CONCLUSIONS: These results show that GCs and IL-10 stimulate GILZ production by human MCs. As GILZ mediates anti-inflammatory effects of GCs in immune cells, we speculate that GILZ could account for the deactivation of MCs by GCs and IL-10. |
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| 1 : | Cytokines, chimiokines et immunopathologie |
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INSERM : U764 – IFR13 – Université Paris XI - Paris Sud |
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| 2 : | Laboratoire Francis PERRIN (LFP) |
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CNRS : URA2453 – CEA : DSM/IRAMIS |
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| 3 : | Centre d'étude des environnements terrestre et planétaires (CETP) |
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CNRS : UMR8639 – INSU – Université de Versailles Saint-Quentin-en-Yvelines |
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| 4 : | Laboratoire de géobiologie, biochronologie et paléontologie humaine (LGBPH) |
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CNRS : UMR6046 – Université de Poitiers |
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| 5 : | Laboratoire pour l'utilisation du rayonnement électromagnétique (LURE) |
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CNRS : UMR130 – CEA – MENRT |
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| 6 : | Laboratoire de Biotechnologie et Pharmacologie génétique Appliquée (LBPA) |
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CNRS : UMR8113 – École normale supérieure de Cachan - ENS Cachan |
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| 7 : | Service de microbiologie, immunologie biologique |
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Assistance publique - Hôpitaux de Paris (AP-HP) – Hôpital Antoine Béclère – Université Paris XI - Paris Sud |
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| 8 : | Laboratoire de Physique et Mécanique Textiles (LPMT) |
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ENSITM – Université de Haute Alsace - Mulhouse |
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| 9 : | Laboratoire d'étude des textures et application aux matériaux (LETAM) |
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CNRS : UMR7078 – Université Paul Verlaine - Metz |
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| Domaine | : | Sciences du Vivant/Immunologie |
| hal-00282496, version 1 | |
| http://hal.archives-ouvertes.fr/hal-00282496 | |
| oai:hal.archives-ouvertes.fr:hal-00282496 | |
| Contributeur : Michel Arock | |
| Soumis le : Mardi 27 Mai 2008, 16:13:03 | |
| Dernière modification le : Jeudi 15 Janvier 2009, 15:55:59 | |
